Open flow microperfusion (OFM) is an in-vivo sampling technique that builds on the strengths of continuous, minimally invasive interstitial sampling methods such as microdialysis (MD). Instead of a membrane, OFM uses probes with macroscopic openings to exchange substances in a liquid pathway that is based on convection rather than diffusion so that the exchange can occur non-selectively in either direction.
The direct liquid pathway between the probe’s perfusate and the surrounding interstitial fluid (ISF) provides a diluted but otherwise unchanged ISF sample, since no dialysis or filtering occurs. OFM provides unfiltered, merely diluted interstitial fluid from the investigated target tissues (skin, adipose, brain tissue). Endogenous and exogenous substances present in the interstitial fluid of targeted tissues can therefore be quantified without the risk of investigating an altered sample.
Transport and effects of drugs in the dermis after topical or systemical application; e. g. topical glucocorticoids and systemic antibodies with psoriasis.
Transport over blood-brain-barrier (BBB), dose-response with bypassing BBB, monitoring of BBB function; e. g. highly lipophilic neuropharmaceuticals.
Transport routes and effects of drugs in adipose tissue, e.g. insulins.
Advantages of cOFM material